Refine your search
Collections
Co-Authors
Journals
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Kshama Devi,
- Antimutagenic Activity of Ashwagandha
Abstract Views :464 |
PDF Views:457
Authors
Source
Journal of Natural Remedies, Vol 5, No 2 (2005), Pagination: 126-131Abstract
Objective: To investigate the protective effect of methanolic extract of Withania somnifera (Ashwagandha) on Mitomycin C induced damage in mouse bone marrow and antioxidant enzymes in liver. Materials and Methods: Protective effect of methanolic extract of Ashwagandha (250 mg/kg b.w. p.o, for 7 days) was assessed against formation of micronuclei (MN) in polychromatic cells (PCEs) and normochromatic cells (NCEs) induced by Mitomycin C (4 mg/kg b.w., i.p) using mouse bone marrow micronucleus test. The effect of Mitomycin C and Ashwagandha extract on liver enzymes, superoxide desmutase (SOD), and glutathione (GSH) was also evaluated. Results: The frequency of occurrence of micronucleated cells in bone marrow was reduced significantly when methanolic extract of Ashwagandha was administered before Mitomycin C. There was a reversal of decrease in P/N ratio. The level of SOD and GSH in liver was significantly depressed by Mitomycin C treatment and the Ashwagandha treatment enhanced level of these enzymes significantly. Conclusions: These findings suggest that Ashwagandha extract is effective in preventing DNA damage, and one of the mechanisms of action might involve scavenging of active oxygen radicals generated in reactions initiated by the mutagens.Keywords
Ashwagandha, Antimutagens, Micronucleus Test, MitomycinFull Text
- Anti-mutagenic Effect of Citral Against Mitomycin-C
Abstract Views :419 |
PDF Views:424
Authors
Source
Journal of Natural Remedies, Vol 6, No 1 (2006), Pagination: 77-82Abstract
Objective : To evaluate the anti-mutagenic activity of citral against the known clastogen mitomycin-C. Materials and methods: Citral was tested at 40 and 80 mg/kg body weight (b.w.) against mitomycin-C (MMC-4 mg/kg, b.w.) using micronucleus test system. Micronucleus (MN) frequency was evaluated in bone marrow and peripheral blood erythrocytes in mice and the sampling was done after 24, 48 and 72 h of clastogen treatment. The antioxidant effect of citral was studied in vitro by super oxide scavenging activity. Results: The results showed that citral significantly (p<0.001) inhibited the MN frequency induced by MMN, further it had exhibited a good super oxide scavenging activity (EC50=19 mg/ml). Conclusion : The observations indicate that continuous presence of citral was essential in minimizing the genotoxic effects of MMC. Besides, it can be suggested that the antioxidant potential of citral was responsible for the anti-mutagenic effect of citral against MMC.Keywords
Citral, Micronucleus Test, Mitomycin-C, AntioxidantFull Text